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食用菌菌种和其他生物一样,具有遗传性和变异性,菌种保存过程中,不仅要保持菌种的成活,更主要的是保持菌种的遗传性状不发生或尽可能少发生变异.过去有些经多年辛勤研究获得的优良菌株,由于缺乏妥善的保存方法而发生变异,失去了原有的优良特性.因此,菌种保存是一项重要的基础工作.目前国际上公认的较为理想的菌种保存方法是液氮超低温保存,但用这种方法保存菌种需要有一定的设备,且液氮来源有限,费用高,所以还不能普及推广使用.笔者根据低温、隔氧可控制微生物繁殖和减慢微生物代谢速度,使它处于休眠或半休眠状态的原理,于1987年开
Edible fungi and other organisms, as with the genetic and variability, strain preservation process, not only to maintain the survival of the species, but more important is to maintain the genetic traits of the strain does not occur or mutated as little as possible. Some excellent strains obtained after years of hard work, due to the lack of proper preservation methods and mutation, loss of the original good characteristics. Therefore, the preservation of bacteria is an important basis for the work. Now internationally recognized as the ideal bacteria The preservation method is liquid nitrogen cryogenic preservation, but this method of preservation of species need to have certain equipment, and liquid nitrogen sources are limited, high cost, so it can not be widely used to promote the author based on the low temperature, oxygen can control microbial propagation and Slow down the metabolic rate of microorganisms, so that it is dormant or semi-dormant state principle, opened in 1987