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水稻组织培养研究,自从1967年取得种子愈伤组织再生植株以来,确有很大进展。先后从花药、子房、未成熟胚乳、根、茎尖、幼穗、枝梗、叶鞘、成熟胚乳等得到植株。还从于房得到单倍体和从胚乳得到三倍体植株。但水稻组织培养尚存在二大难题:一是未能从叶片诱导产生愈伤组织,并将它再生成植株;二是从原生质诱发产生的愈伤组织也未能再生成植株。为此,我们进行了叶片组织培养研究,以期证实叶细胞的全能性,提高愈伤组织再生成植株的培养技术。一、材料方法实验采用粳稻双丰1号、嘉农15、寒丰、花寒早;籼稻广陆矮4号、原丰早;杂交种
Research on tissue culture in rice has made great strides since the plant was regenerated from seed calluses in 1967. From anther, ovary, immature endosperm, root, shoot tip, young spike, branch stem, leaf sheath, mature endosperm and other plants. Also obtained from the room haploid and triploid plants obtained from the endosperm. However, there are still two major problems in tissue culture of rice: one is the inability to induce callus from leaf and regenerate it into plants; the other is that callus induced from protoplasts failed to regenerate into plants. To this end, we conducted a leaf tissue culture research to confirm the pluripotency of the leaf cells and improve the callus regeneration of plant culture techniques. First, the material method experiments using japonica rice Shuangfeng No. 1, Jianong 15, cold Feng, Han Han-early; Guanglu Dwarf 4 indica early morning; hybrids