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建立基于半胱氨酸调控量子点合成的新型荧光传感器用于Cu~(2+)的快速、灵敏检测。以半胱氨酸为稳定剂,可在室温条件快速原位合成水溶性的荧光量子点。通过Cu~(2+)催化半胱氨酸氧化而抑制量子点的生长。由于催化形成的胱氨酸产物失去有效的稳定作用,导致合成的量子点数量减少,从而实现Cu~(2+)的荧光检测。在5~400 nmol/L范围内,Cu~(2+)浓度与荧光信号呈良好的线性关系,检出限为4.2 nmol/L。该传感器对Cu~(2+)具有良好选择性。用于闽江水中Cu~(2+)的测定,结果与ICP-MS方法接近。所建立的传感器具有操作简便、成本低及快速等优点。整个传感过程在15 min内即可完成。因此,在其它多种目标分析物的间接检测中具有广泛的应用前景。
A novel fluorescence sensor based on cysteine-regulated quantum dot synthesis was established for the rapid and sensitive detection of Cu 2+. To cysteine as a stabilizer, at room temperature conditions can be quickly synthesized in situ water-soluble fluorescent quantum dots. Cu (2+) catalyzes the oxidation of cysteine to inhibit the growth of quantum dots. Due to the loss of effective stabilizing effect of the catalyzed cystine product, the amount of synthesized quantum dots is reduced, thereby realizing the fluorescence detection of Cu 2+. In the range of 5 ~ 400 nmol / L, the concentration of Cu 2+ showed a good linear relationship with the fluorescence signal, with a detection limit of 4.2 nmol / L. The sensor has good selectivity for Cu ~ (2+). It is used for the determination of Cu ~ (2+) in Minjiang River water and the result is close to ICP-MS method. The sensor is easy to operate, low cost and fast. The entire sensing process can be completed within 15 min. Therefore, it has a wide range of applications in the indirect detection of many other target analytes.