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目的探讨ACK1在肺鳞状细胞癌组织中的表达及其临床意义。方法分别采用RT-PCR、免疫组化法检测45名患者肺鳞状细胞癌及癌旁正常黏膜组织标本中ACK1基因mRNA的转录水平和ACK1蛋白的表达水平,并分析ACK1高表达与临床病理特征之间的相关性。结果 60%的肺鳞状细胞癌组织中ACK1基因mRNA转录水平升高;64.4%的肺鳞状细胞癌细胞浆中ACK1蛋白高表达;ACK1高表达与肺鳞状细胞癌的淋巴结转移、肿瘤分期相关(P<0.05);ACK1高表达与肺鳞状细胞癌的不良预后相关,但不是一个独立的影响预后的因素。结论 ACK1可作为一种新型分子标记物,预测原发性肺鳞状细胞癌患者的肿瘤侵袭和淋巴结转移,判断患者预后转归,为肿瘤的个体化治疗提供了实验依据。
Objective To investigate the expression of ACK1 in lung squamous cell carcinoma and its clinical significance. Methods RT-PCR and immunohistochemistry were used to detect the mRNA level of ACK1 mRNA and the expression of ACK1 protein in lung squamous cell carcinoma and adjacent normal mucosa tissues of 45 patients. The expression of ACK1 and its clinicopathological features The correlation between. Results The mRNA level of ACK1 gene in 60% of lung squamous cell carcinoma tissues was increased, 64.4% of the lung squamous cell carcinoma tissues had high expression of ACK1 protein, and the high expression of ACK1 was associated with lymph node metastasis of lung squamous cell carcinoma, (P <0.05). The high expression of ACK1 was associated with poor prognosis of lung squamous cell carcinoma, but not an independent prognostic factor. Conclusion ACK1 can be used as a new molecular marker to predict tumor invasion and lymph node metastasis in patients with primary lung squamous cell carcinoma and to judge prognosis of patients with lung squamous cell carcinoma and provide an experimental basis for the individualized treatment of tumors.