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目的 :探讨完全弗氏佐剂 (CFA)、不完全弗氏佐剂 (IFA)、大复层脂质体 (LML)、小单层阳离子脂质体(DOTAP)对NOD鼠胰岛炎和糖尿病发病的影响。方法 :①将 3周龄的NOD雌鼠随机分为 3组 ,分别给予CFA后脚板注射 (n =16 ) ,IFA和PBS乳化后腹腔注射 (n =16 ) ,单独PBS后脚板注射 (n =16 )。观察 12周龄胰岛病理及 30周龄糖尿病发病情况。②将 3周龄的NOD雌鼠随机分为 3组 ,分别给予大复层脂质体 (LML) (n =13) ,小单层阳离子脂质体 (DOTAP) (n =13) ,单独PBS腹腔注射 (n =13) ,同上观察胰岛病理及糖尿病发病情况。结果 :①CFA后脚板注射能明显降低胰岛炎计分 ,减少糖尿病的发生。 30周龄时 ,CFA组的发病率明显低于PBS后脚板注射对照组 (分别为9 2 %和 81 8% ,P =0 .0 0 1)。 12周龄时 ,CFA组胰岛炎计分明显低于对照组 [分别为 (0 0 5± 0 0 2 )和 (0 5 4± 0 11) ,P<0 .0 0 1]。②LML腹腔注射能明显降低胰岛炎计分。 12周龄时 ,LML组胰岛炎计分明显低于PBS腹腔注射对照组 [分别为 (0 16± 0 0 2 )和 (0 5 8± 0 0 6 ) ,P <0 .0 0 1]。 30周龄时 ,LML组糖尿病发生率为 6 0 % ,对照组为 90 % ,两组间无统计学差异。③在预防胰岛炎的作用上 ,CFA优于LML。结论 :CFA后脚板注射可预防NOD鼠发生糖尿病并减?
OBJECTIVE: To investigate the effects of complete Freund’s adjuvant (CFA), incomplete Freund’s adjuvant (IFA), large complex liposomes (LML) and small single-layer cationic liposomes (DOTAP) on insulitis and diabetes mellitus Impact. Methods: ① 3-week-old NOD female rats were randomly divided into three groups. The CFA posterior platelet injection (n = 16), IFA and PBS intraperitoneal injection (n = 16) 16). The 12-week islet pathology and 30-week-old diabetic patients were observed. (2) Three-week-old NOD female mice were randomly divided into three groups: large-lamellar liposome (LML) (n = 13), small single-layer cationic liposome (DOTAP) Intraperitoneal injection (n = 13), ibid view islet pathology and diabetes incidence. Results: ①CFA posterior pedicle injection can significantly reduce insulitis score, reduce the incidence of diabetes. At 30 weeks of age, the incidence of CFA was significantly lower than that injected into the posterior plate of PBS (92% and 81 8%, respectively, P = .0101). At 12 weeks of age, the scores of insulitis in CFA group were significantly lower than those in control group [(0 05 ± 0 0 2) and (0 54 ± 0 11, respectively; P 0 01). ② LML intraperitoneal injection can significantly reduce the score of insulitis. At 12 weeks of age, the scores of insulitis in LML group were significantly lower than those in PBS intraperitoneal injection group [(0 16 ± 0 0 2) and (0 58 ± 0 0 6), respectively; P 0 01]. At 30 weeks of age, the incidence of diabetes was 60% in the LML group and 90% in the control group, with no significant difference between the two groups. ③ In the role of prevention of insulitis, CFA is better than LML. Conclusion: CFA posterior platelet injection can prevent diabetic NOD mice and reduce?