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目的: 研究电磁脉冲对大鼠海马组织的损伤效应及损伤相关因子表达情况。方法: 海马组织切片采用HE染色及甲苯胺兰染色后光镜下观察病理学改变; SP免疫组织化学染色法检测Bcl-2、GFAP、c fos的表达。结果6×104V/m照射后1h海马神经元呈不同程度变性, 胞浆尼氏体普遍减少, 神经元固缩。Bcl-2蛋白表达于照后1h升高显著(P<0 .05), 6~24h下降并低于对照组。GFAP于照后1h即开始上调, 6~24h明显升高(P<0 .05)。c fos于EMP照射后呈持续性增高, 6~24h升高显著, 其中12h达峰值(P<0 .01)。结论: EMP照射导致大鼠海马神经元的损伤, Bcl-2、GFAP、c -fos因子的表达参与了EMP所致的损伤及修复机制。
Objective: To study the effect of electromagnetic pulse on the damage of rat hippocampus and the expression of injury related factors. Methods: The sections of hippocampus were stained with HE staining and toluidine blue staining and the pathological changes were observed under light microscope. The expressions of Bcl-2, GFAP and c fos were detected by SP immunohistochemical staining. Results The hippocampal neurons degenerated to varying degrees 1 h after 6 × 104 V / m irradiation, with a general decrease of Nissl and a shrinkage of neurons. The expression of Bcl-2 protein increased significantly at 1h after irradiation (P <0.05), decreased at 6 ~ 24h and was lower than that of control group. GFAP began to rise at 1h after irradiation and was significantly increased from 6 to 24 hours (P <0.05). c fos was continuously increased after EMP irradiation and increased significantly at 6 ~ 24h, with a peak at 12h (P <0.01). CONCLUSIONS: The injury of hippocampal neurons induced by EMP irradiation and the expression of Bcl-2, GFAP and c-fos are involved in the injury and repair mechanisms induced by EMP.