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目的观察脓毒症小鼠外周血单核细胞程序性细胞死亡因子配体1(PD-L1)的表达及其在轻型、重型脓毒症组间的差异。方法 30只C57小鼠随机分为轻型脓毒症组、重型脓毒症组及假手术组,每组10只,采用盲肠结扎穿孔法造模,以盲肠结扎长短区分病情轻重,造模成功后观察生存率。60只C57小鼠随机分为轻型脓毒症组、重型脓毒症组及假手术组,每组均为20只。分别在造模后6 h、24 h时间点取外周血,每个时间点10只。流式细胞技术检测各组单核细胞PD-L1的表达,酶联免疫吸附法检测血清肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)水平。结果重型脓毒症组较轻型脓毒症组死亡率高。6 h、24 h脓毒症组血清TNF-α和IL-10水平较假手术组明显升高(P<0.01)。6 h和24 h脓毒症组单核细胞PD-L1表达较假手术组升高(P<0.05),24 h脓毒症组PD-L1表达升高更明显(P<0.01)。重型脓毒症组PD-L1表达较轻型脓毒症组有升高趋势,但组间差异无统计学意义(P>0.05)。结论脓毒症小鼠单核细胞PD-L1高表达,重型脓毒症组较轻型脓毒症组有升高趋势。
Objective To observe the expression of PD-L1 in peripheral blood mononuclear cells (PBMCs) of septic mice and its difference between the light and severe sepsis groups. Methods Thirty C57 mice were randomly divided into mild sepsis group, severe sepsis group and sham operation group, 10 rats in each group. The cecal ligation and perforation method was used to establish the model. The severity of cecal ligation was discriminated after the model was established Survival rate was observed. Sixty C57 mice were randomly divided into mild sepsis group, severe sepsis group and sham operation group, with 20 in each group. Peripheral blood samples were taken at 6 h and 24 h after modeling, respectively, at 10 time points. Flow cytometry was used to detect the expression of PD-L1 in each group. Serum levels of TNF-α and IL-10 were detected by enzyme-linked immunosorbent assay. Results In severe sepsis group, the mortality rate was higher in patients with mild sepsis. Serum levels of TNF-α and IL-10 in 6 h and 24 h sepsis group were significantly higher than those in sham operation group (P <0.01). The PD-L1 expression of monocytes in sepsis group was significantly higher than that in sham operation group at 6 h and 24 h (P <0.05), and the PD-L1 expression in sepsis group increased significantly at 24 h (P <0.01). The expression of PD-L1 in severe sepsis group tended to be higher than that of mild sepsis group, but there was no significant difference between the two groups (P> 0.05). Conclusions The expression of PD-L1 in monocytes of sepsis mice is high, but in severe sepsis patients there is a trend of increase.