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目的：初步建立并优化用于蛋白质组分析的双向电泳技术，提高其分辨率及重复性。方法：以ＨｅｐＧ２细胞为例，对以固相ｐＨ梯度为第一向的双向电泳的关键因素与环节，如样品处理、电泳参数和凝胶浓度进行一系列的优化。结果与结论：本研究采用增溶、排异的裂解法处理样品，选择适中的样品量，用预制固相ｐＨ梯度——ＩＰＧ胶条（ｐＨ＝３～１０）第进行第一向等电聚焦，并选用１２％～１４％的梯度胶进行第二向ＳＤＳ分离，可获得质量较好的双向电泳图谱
OBJECTIVE: To initially establish and optimize two-dimensional electrophoresis for proteomic analysis to improve its resolution and reproducibility. Methods: Taking HepG2 cells as an example, a series of optimization of key factors and links of two-dimensional electrophoresis, such as sample processing, electrophoresis parameters and gel concentration, were carried out. RESULTS AND CONCLUSION: In this study, the samples were treated by solubilization and rejection method. The samples were selected moderately, and the first-dimension isoelectric focusing was carried out by pre-solid phase pH gradient - IPG strips (pH = 3-10) , And the selection of 12% to 14% of the gradient gel for the second SDS separation, get better quality two-dimensional electrophoresis