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报道了λ噬菌体在大肠杆菌W1485中增殖、裂解的条件。用5′-磷酸二酯酶与SephadexG-200凝胶过滤纯化的λDNA,在琼脂糖凝胶呈一条带。经自行纯化的EcoRⅠ限制性内切酶切割后,琼脂糖凝胶电泳上出现6个片段,证明了该酶对它具有5个切点
The conditions for proliferation and lysis of lambda phage in E. coli W1485 were reported. The purified λDNA was gel-filtered with 5’-phosphodiesterase and Sephadex G-200, showing a band on the agarose gel. After purification by EcoR I restriction endonuclease cleavage, the agarose gel electrophoresis showed six fragments, it has to prove that the enzyme has 5 points