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本研究采用RAPD和AFLP方法对 2 3个中国不同地区的食用向日葵 (HelianthusannuusL .)骨干品种进行了遗传变异分析 ,同时对两种标记系统进行了比较。 2 6个RAPD引物产生了总计 192条DNA条带 ,大小分布于0 .2 6kb～ 1.98kb之间 ,其中 16 5条 (86 .12 % )具有多态性 ,每条引物产生DNA条带的平均数为 7.38。 8对AFLP引物组合共产生了 5 76条带 ,分布于 10 0bp～ 5 0 0bp之间 ,其中的 341条具有多态性 ,多态百分率为 76 .0 0 % ,每对引物组合产生DNA条带的平均数为 72。RAPD方法检测到的每位点有效等位基因数 (1.76 )大于AFLP(1.6 5 ) ,AFLP标记位点的平均多态性信息量 (PIC) (0 .38)低于RAPD标记位点的PIC(0 .4 1) ,但AFLP标记具有很高的多态性检测效率(Ai=38.5 2 )。用RAPD标记分析 2 3个食用向日葵材料的亲缘关系 ,Nei氏相似性系数分布在 4 7.84 %～ 82 .0 6 % ,平均相似性系数为 0 .6 4 95 ,而采用AFLP的Nei氏相似性系数分布在 5 4 .15 %～ 83.5 2 % ,平均相似性系数为 0 .6 884。RAPD数据的标准差为 0 .13,而AFLP数据的标准差为 0 .0 8。因此 ,采用RAPD和AFLP方法分析食用向日葵遗传变异 ,RAPD标记具有较低相似性系数和较高方差而AFLP则相反。源于两种不同标记的遗传相似性矩阵的相关系数为 0 .5 1,说明
In this study, genetic variation analysis was conducted on 23 key cultivars of Helianthus annuus L.in different regions of China using RAPD and AFLP methods, and the two marker systems were compared. A total of 192 DNA bands were generated by 6 RAPD primers, ranging in size from 0.226kb to 1.98kb, of which 165 (86.12%) were polymorphic and each primer had DNA bands The average is 7.38. 8 pairs of AFLP primer combinations produced a total of 5 76 bands, located in the 10 0bp ~ 500bp, of which 341 were polymorphic, the percentage of polymorphisms was 76.0%, each pair of primers to produce DNA strips The average number of bands is 72. The number of effective alleles per locus (1.76) detected by RAPD method was greater than that of AFLP (1.6 5), PIC of AFLP loci (0.38) was lower than that of RAPD loci (0.41), but the AFLP marker has high polymorphism detection efficiency (Ai = 38.5 2). The RAPD markers were used to analyze the genetic relationship of 23 edible sunflower materials. The Nei’s similarity coefficient ranged from 4 7.84% to 82.06% with an average similarity coefficient of 0.6495. The Nei’s similarity of AFLP The coefficients ranged from 54.15% to 83.5% and the average similarity coefficient was 0.6884. The standard deviation of RAPD data was 0.13, while the standard deviation of AFLP data was 0.08. Therefore, RAPD and AFLP analysis of edible sunflower genetic variation, RAPD markers with lower similarity coefficient and higher variance AFLP is the opposite. The correlation coefficient of the genetic similarity matrix from the two different markers is 0.51, indicating