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为了明确迁移和基因交流在杀虫剂抗性基因进化中的作用 ,我们从四个不同的地区采集有机磷抗性的库蚊野生种群 ,利用淀粉电泳鉴定了各种群中存在的已知过量产生酯酶的分布频率 ,并通过 5个假定的中性位点的电泳多态性分析了种群间的遗传多样性。结果表明种群间的基因交流是存在的 ,遗传分化与地理位置存在一定关系 ,而抗性等位基因A2 B2的分布却与种群间的遗传分化不一致。对这种差异的解释是 :被动迁移 (铁路运输等 )加速了抗性基因的交流 ,而当抗性基因以自然迁飞的方式向周围地区扩散时 ,却是一个相对缓慢的过程。
To clarify the role of migration and gene exchange in the evolution of pesticidal resistance genes, we collected organophosphate-resistant populations of Culex pipiens from four different areas and identified the presence of known over-abundances in each population using starch electrophoresis The esterase distribution frequency was generated and the genetic diversity among populations was analyzed by electrophoretic polymorphisms at five putative neutral sites. The results showed that there was gene exchange among populations. Genetic differentiation had a certain relationship with geographical location, but the distribution of resistant allele A2 B2 was not consistent with the genetic differentiation among populations. The explanation for this difference is that passive migration (rail transport, etc.) accelerates the exchange of resistance genes and is a relatively slow process when resistant genes are diffused into the surrounding area by natural migration.