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在改良MS培养基上 ,全黑暗条件下培养未经任何处理的玉米种子萌发的茎尖 ,可诱导雌雄花序的发生 .培养基 6 BA浓度 2 0mg/L时 ,自交系 5 15 ,8112及其杂交后代的茎尖分生组织经过 5～ 6周的培养 ,可直接分化产生雌雄花序 ;培养基的激素组合为 6 BA 1.0mg/L +2 ,4 D 0 .2mg/L ,供试的 6个自交系的茎尖膨大生长 ,产生较多的丛生芽 ,在转入添加 6 BA 1.0mg/L +IBA 0 .2mg/L的培养基上后 ,培养物在继续产生丛生芽的同时 ,分化出大量的雌雄花序 .雌、雄花序发生过程与田间植株的基本相同 ,但雌穗远多于雄穗 .适宜外源激素是诱导雌、雄花序发生所必需的 ,基因型决定了外植体对诱导条件的反应 .由于离体茎尖能直接产生生殖器官 ,推测玉米营养生长和生殖器官发生被不同调控机制所控制 .
On the modified MS medium, the shoots of maize seeds germinated without any treatment could induce the emergence of male and female inflorescences under the condition of darkness.When the concentration of BA was 20 mg / L, the inbred lines 5 15, 8112 and The shoot apical meristem of its hybrid progeny could be directly differentiated to produce male and female inflorescences after culturing for 5 to 6 weeks. The hormone combinations in the medium were 6 BA 1.0 mg / L +2 and 4 D0.2 mg / L, The stem tips of six inbred lines expanded and produced more cluster buds. After transferring into the medium supplemented with 6 BA 1.0 mg / L and IBA 0.2 mg / L, the culture continued to produce clumping buds , A large number of male and female inflorescences were differentiated.The process of female and male inflorescences was basically the same as that of field plants, but the number of female inflorescences was much more than that of male ones.The suitable exogenous hormones were necessary for inducing female and male inflorescences, and the genotypes determined The response of explants to the induction conditions.Because the ex vivo stem tips can directly produce genital organs, it is speculated that the vegetative growth and reproductive organs of maize are controlled by different regulatory mechanisms.