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目的:研究戊四氮诱导的大鼠癫痫持续状态模型(status epilepticus,SE)中,P-糖蛋白(P-glycoprotein,P-gp)在72 h内表达的动态变化,为下一步抑制P-gp的表达选定最佳检测时间点。方法:用免疫组化、RT-q PCR和Western blot检测建模后不同时点(0、3、6、12、24、48、72 h)海马组织中P-gp表达的变化情况。结果:免疫组化结果:海马组织中P-gp蛋白在SE 24 h组的平均光密度值为0.325 1±0.008 2,比对照组增加(P<0.05),SE 48 h组的平均光密度值为0.396 3±0.016 8,显著高于对照组(P<0.01);RT-q PCR结果:MDR1a基因在SE 24 h组比对照组增加(P<0.05),SE 48 h组显著高于对照组(P<0.01);Western blot结果:P-gp蛋白在SE 48 h组比对照组增加(P<0.05)。这些结果显示,SE模型中海马组织内P-gp表达在24 h后开始升高,并在48 h达高峰。结论:SE后48 h P-gp的表达达最高峰,此时可能为减少SE后产生耐药的最佳检测时间。
PURPOSE: To investigate the dynamic changes of P-glycoprotein (P-glycoprotein) expression in status epilepticus (SE) induced by pentylenetetrazol in rats for 72 h, gp expression selected the best detection time point. Methods: The expression of P-gp in hippocampus was detected by immunohistochemistry, RT-q PCR and Western blot at different time points (0, 3, 6, 12, 24, 48, 72 h) Results: The average optical density of P-gp protein in SE 24 h hippocampus was 0.325 1 ± 0.008 2, which was higher than that in control group (P <0.05). The average optical density value of SE 48 h group (0.396 3 ± 0.016 8, P <0.01). RT-qPCR results showed that the expression of MDR1a gene in SE 24 h group was higher than that in control group (P <0.05), and significantly higher in SE 48 h group than that in control group (P <0.01). Western blot results showed that P-gp protein increased in SE 48 h group compared with control group (P 0. 05). These results show that the expression of P-gp in hippocampus of SE model began to increase after 24 h and peaked at 48 h. Conclusion: The peak expression of P-gp at 48 h after SE may be the best detection time to reduce the drug resistance after SE.