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本研究探讨丙戊酸钠(SVPA)协同阿霉素(ADM)抑制骨髓增生异常综合征细胞株MUTZ-1增殖并诱导其凋亡的作用。采用细胞形态学方法观察小剂量SVPA和ADM处理后细胞形态的改变,应用(MTT)比色法检测SVPA及ADM对细胞生长的抑制作用,流式细胞术分析细胞凋亡。结果表明:不同浓度的ADM(0.039μg/ml、0·078μg/ml、0.156μg/ml、0.312μg/ml和0.4μg/ml)与0.25mmol/LSVPA联合作用于MUTZ-1细胞72小时后,细胞抑制率分别为(23.46±1.12)%、(49.87±0.84)%、(52.37±1.05)%、(78.43±4.34)%和(82.47±1·04)%,明显高于单用ADM时的(5.08±0.79)%、(12.32±2.39)%、(23.65±1.34)%、(43.33±2.38)%和(47.85±1·46)%(p<0.05)。单用0.25mmol/LSVPA时对细胞生长无影响(p>0.05)。SVPA联合ADM作用细胞株72小时后,细胞呈现典型的凋亡细胞的形态学特征;SVPA和ADM联合作用后细胞的凋亡率比单独使用ADM明显增加,并呈浓度依赖性,与对照组相比有显著的统计学意义,其中以0.078μg/ml浓度ADM为最佳抑制浓度。结论:小剂量的SVPA能增敏ADM诱导骨髓增生异常综合征细胞株MUTZ-1发生凋亡。
This study was to investigate the effect of sodium valproate (SVPA) in combination with doxorubicin (ADM) on the proliferation and apoptosis of myelodysplastic syndrome cell line MUTZ-1. Cell morphology was used to observe the changes of cell morphology after treatment with SVPS and ADM. MTT assay was used to detect the inhibitory effects of SVPA and ADM on cell growth. Flow cytometry was used to analyze the apoptosis. The results showed that MUTZ-1 cells were treated with different concentrations of ADM (0.039μg / ml, 0.078μg / ml, 0.156μg / ml, 0.312μg / ml and 0.4μg / ml) The cell inhibitory rates were (23.46 ± 1.12)%, (49.87 ± 0.84)%, (52.37 ± 1.05)%, (78.43 ± 4.34)% and (82.47 ± 1.40)%, respectively, (5.08 ± 0.79)%, (12.32 ± 2.39)%, (23.65 ± 1.34)%, (43.33 ± 2.38)% and (47.85 ± 1.46)%, respectively. With 0.25mmol / LSVPA alone had no effect on cell growth (p> 0.05). After 72 hours SVCA combined with ADM-induced cell line, the cells showed the morphological characteristics of typical apoptotic cells. The apoptosis rate of SVPA combined with ADM was significantly higher than that of ADM alone and in a concentration-dependent manner. Compared with control group There was significant statistical significance, with 0.078μg / ml ADM concentration as the best concentration. CONCLUSIONS: SVPA can sensitize ADM cells to induce apoptosis of myelodysplastic syndrome cell line MUTZ-1.