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目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应的Ct值分别在25.82～25.88,29.01～29.08,15.52～15.56,19.06～19.08变化,SD分别是0.033,0.032,0.024,0.011,其CV分别是0.12%,0.22%,0.16%,0.06%。结论:所建立的Real time PCR体系重复性好,检测系统工作稳定可靠,可用于甘草HMGR,SQS1,β-AS基因的CNVs筛选。
OBJECTIVE: To establish a stable and reliable detection system for the determination of CNGRs of licorice HMGR, SQS1 and β-AS synthase genes. Methods: The Real Time PCR (PCR) was used to determine the CNVs of licorice HMGR, SQS1 and β-AS synthase genes. Results: In the quantitative test of HMGR, SQS1, β-AS synthase gene and lectin, the Ct values of quantitative reaction were changed from 25.82 ~ 25.88, 29.01 ~ 29.08, 15.52 ~ 15.56 and 19.06 ~ 19.08 respectively. SD Respectively 0.033,0.032,0.024,0.011, the CV is 0.12%, 0.22%, 0.16%, 0.06% respectively. Conclusion: Real-time PCR system established has good repeatability and stable and reliable detection system. It can be used for CNV screening of licorice HMGR, SQS1 and β-AS genes.