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采用ＲＴＰＣＲ技术扩增大鼠ＯＢｃＤＮＡ编码区序列。ＰＣＲ产物酶切后定向克隆至ｐＵＣ１９质粒。经核苷酸序列测定表明与文献报道的大鼠ＯＢｃＤＮＡ编码区序列一致。继之构建了ｐＢＶ２２０ｒＯＢ表达质粒并获得了ＯＢ基因在大肠杆菌中的特异表达。大鼠ＯＢ基因产物的获得为研究肥胖与某些非传染性疾病（如糖尿病、高血压病）间的关系提供了条件
The RT-PCR technique was used to amplify the coding region of rat OBcDNA. The PCR product was digested and cloned into the pUC19 plasmid. Nucleotide sequence analysis showed that the coding sequence of OBcDNA in rat was consistent with that reported in the literature. Followed by the construction of pBV220 rOB expression plasmid and OB gene was obtained in E. coli specific expression. The availability of rat OB gene products provides the basis for studying the relationship between obesity and certain noncommunicable diseases such as diabetes and hypertension