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[Objective]To investigate the feasibility of Viola inconspicus Bl.using as the plant origin of HERBA VIOLAE.[Methods]L9(33)orthogonal test was adopted.With the content of total flavonoids as the evaluation index,effects of ethanol concentration,ethanol dosage and extraction time on the content of total flavonoids were researched.Under the optimal extraction technology,the content of total flavonoids was detected in V.inconspicus,and the result was compared with that in HERBA VIOLAE.[Results]The optimal extraction condition was as follows:1∶40 solid-liquid ratio(g∶mL),80%ethanol and 40 min extraction time.Among the different parts of V.inconspicus,flowers had the highest content of total flavonoids,followed with leaves.Compared with Zigong April C,HERBA VIOLAE had slightly higher content of total flavonoids,and there was only small difference between the two.[Conclusions]V.inconspicus could be used as the plant origin of HERBA VIOLAE.The above-ground part of V.inconspicus was harvested and the optimal harvesting time was flowering period.
[Objective] To investigate the feasibility of Viola inconspicus Bl. Using as the plant origin of HERBA VIOLAE. [Methods] L9 (33) orthogonal test was adopted. What the content of total flavonoids as the evaluation index, effects of ethanol concentration, ethanol dosage and extraction time on the content of total flavonoids were researched.Under the optimal extraction technology, the content of total flavonoids was detected in V.inconspicus, and the result was compared with that in HERBA VIOLAE. [Results] The optimal extraction condition was as follows: 1:40 solid-liquid ratio (g: mL), 80% ethanol and 40 min extraction time. Among the different parts of V. inconspicus, flowers had the highest content of total flavonoids, followed by leaves. Compared with Zigong April C, HERBA VIOLAE had slightly higher content of total flavonoids, and there was only small difference between the two. [Conclusions] V. inconspicus could be used as the plant origin of HERBA VIOLAE. Above-ground part of V. inconspicus was harves ted and the optimal harvesting time was flowering period.