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目的:研究菊明提取物中降压活性物质黄酮类成分的质量控制标准。方法:以蒙花苷和野菊花对照药材为对照品,采用薄层色谱法(TLC)鉴别方中野菊花。以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法测定总黄酮含量;采用高效液相色谱法(HPLC)测定蒙花苷含量,Discovery C18(250mm×4.6mm,5μm),流动相为甲醇-2%醋酸(52:48),检测波长为334nm。结果:薄层斑点清晰,专属性强。芦丁对照品线性范围在13.37-66.84μg/mL,回归方程为Y=0.0129X-0.0116,r=0.9999;平均回收率为98.28%,RSD为2.05%。蒙花苷对照品线性范围在21.90-1 752.00μg/mL,回归方程为Y=92845X+401863,r=0.9999;平均回收率为101.83%,RSD为1.10%。结论:建立的薄层鉴别和含量测定方法简便可行、结果准确,可用于菊明提取物中黄酮类成分的质量控制。
OBJECTIVE: To study the quality control standards of flavonoids in hypotensive active substances in extract of Radix extract. Methods: Monatin and wild chrysanthemum control herbs as reference substance, thin layer chromatography (TLC) Fang Fang Chrysanthemum identification. The content of total flavonoids was determined by using sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method with rutin as reference substance. The content of monatin was determined by high performance liquid chromatography (HPLC), and Discovery C18 (250mm × 4.6mm, 5μm ), The mobile phase is methanol-2% acetic acid (52:48) and the detection wavelength is 334nm. Results: Thin spots clear, specific and strong. The linear range of rutin was 13.37-66.84μg / mL. The regression equation was Y = 0.0129X-0.0116, r = 0.9999. The average recovery was 98.28% and the RSD was 2.05%. The linear range of glycoside reference substance was 21.90-1 752.00μg / mL, the regression equation was Y = 92845X + 401863, r = 0.9999; the average recovery was 101.83% and the RSD was 1.10%. Conclusion: The TLC identification and determination methods are simple and feasible, and the results are accurate and can be used for the quality control of flavonoids in the extract of Radix.