论文部分内容阅读
目的了解近年来深圳市家蝇的抗药性发展状况,探讨大头金蝇对几种常用杀虫剂的抗性,为防制工作提供科学依据。方法采集深圳市区家蝇及大头金蝇,挑选实验室饲养1～2代后的雌性成虫,采用微量点滴法测定其对溴氰菊酯、高效氯氰菊酯、氯菊酯、胺菊酯、DDVP和仲丁威6种杀虫剂的LD50(μl/虫)。结果家蝇对溴氰菊酯、氯菊酯、胺菊酯、DDVP、仲丁威的LD50分别为0.024819、0.092076、0.649075、0.065610和5.008337μg/虫,与1998年的抗性监测结果相比,分别增长8.27、7.25、3.45、0.11和0.54倍;大头金蝇对溴氰菊酯、高效氯氰菊酯、氯菊酯、胺菊酯、DDVP和仲丁威的LD50分别为0.271852、0.318642、0.174697、0.657885、0.086490和1.743459μg/虫。结论家蝇对溴氰菊酯、氯菊酯、胺菊酯的抗性水平均有不同程度升高,但抗性增长速度较为缓慢,对DDVP与仲丁威的抗性呈现下降趋势;大头金蝇的抗性测定结果与家蝇有差异,需改进实验方法并制定相应的抗性级别标准。
Objective To understand the development of drug resistance in housefly in Shenzhen in recent years and to explore the resistance of the common gold flies to several commonly used insecticides to provide a scientific basis for prevention and control. Methods Houseflies and golden needle flies were collected from Shenzhen. The female adults in the laboratory were cultured for 1-2 generations. The contents of deltamethrin, beta-cypermethrin, permethrin, tetramethrin, DDVP and Ding Ding Wei 6 kinds of pesticides LD50 (μl / worm). Results The LD50 of housefly to deltamethrin, permethrin, tetramethrin, DDVP and sec-butyl were 0.024819,0.092076,0.649075,0.065610 and 5.008337μg / worm, respectively. Compared with the results of resistance monitoring in 1998, Respectively increased by 8.27, 7.25, 3.45, 0.11 and 0.54 times respectively. LD50 of deltamethrin to deltamethrin, beta-cypermethrin, permethrin, tetramethrin, DDVP and sec-butylwere were 0.271852,0.318642,0.174697,0.657885, 0.086490 and 1.743459 μg / worm. Conclusion The resistance level of housefly to deltamethrin, permethrin and tetramethrin all increased to some extent, but the rate of resistance increase was relatively slow, and the resistance to DDVP and sec-butyl was decreased. The test results of flies are different from that of house flies, and the experimental methods need to be improved and the corresponding standard of resistance level should be established.