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目的：研究ｎｍ２３－Ｈ１和ｐ５３基因表达与肝细胞癌（肝癌）临床病理学特征的关系。方法：采用ＡＢＣ免疫组织化学染色法检测了２９例肝癌标本的ｎｍ２３－Ｈ１和ｐ５３基因表达。结果：ｎｍ２３－Ｈ１基因在肝癌中表达的阴性率为６０％（１８／２９）；ｎｍ２３－Ｈ１基因低表达与门静脉癌栓、肝癌分化程度Ｅｄｍｏｎｄｓｏｎ分级、血清ＡＦＰ水平等显著相关（Ｐ均＜０．０５）；ｐ５３基因表达阳性率为７０％（２０／２９）；ｐ５３基因过表达与肝癌门静脉癌栓、血清ＡＦＰ水平显著相关（Ｐ均＜０．０５）。结论：（１）ｎｍ２３－Ｈ１基因低表达和ｐ５３基因过表达可能与ＡＦＰ基因激活有关；（２）ｎｍ２３－Ｈ１和ｐ５３基因突变可能促进肝癌门静脉转移。
Objective: To investigate the relationship between the expression of nm23-H1 and p53 genes and the clinicopathological features of hepatocellular carcinoma (liver cancer). Methods: The expression of nm23-H1 and p53 genes in 29 specimens of liver cancer was detected by ABC immunohistochemical staining. Results: The negative rate of nm23-H1 gene expression in liver cancer was 60% (18/29). The low expression of nm23-H1 gene was significantly correlated with portal vein tumor thrombosis, Edmondson grade of liver cancer differentiation, and serum AFP levels (P<0 .05); p53 gene expression positive rate was 70% (20/29); p53 gene overexpression was significantly associated with portal vein tumor thrombosis and serum AFP levels (P < 0.05). Conclusions: (1) The low expression of nm23-H1 gene and overexpression of p53 gene may be related to the activation of AFP gene; (2) The mutation of nm23-H1 and p53 may promote the portal vein metastasis of hepatocellular carcinoma.