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染色体11q23的混合谱系白血病(MLL)基因的断裂点簇集群区(BCR)的转位,可引起婴儿急性白血病及与DNA拓扑异构酶Ⅱ抑制剂的生化治疗法相关的白血病。由于MLL基因包括大约30个不同的转位伴侣基因,几个断裂点所在的伴侣基因的序列还不清楚,因而对MLL基因断裂点的PCR克隆是很困难的。锅柄式PCR,即用已知5’端序列和未知的3’端伴侣基因序列以形似一个锅和一个柄的模板扩增断裂点基因DNA,是一种克隆MLL基因断裂区的新方法,可以扩增未知3’端序列的断裂点簇集群区。
The translocation of the rupture point cluster cluster (BCR) of the mixed lineage leukemia (MLL) gene on chromosome 11q23 can cause leukemia in infants with acute leukemia and biochemotherapy with DNA topoisomerase II inhibitors. Since the MLL gene includes approximately 30 different transplaceable partner genes, the sequence of the chaperone gene at several breakpoints is unclear and PCR cloning of the MLL gene disruption point is difficult. Pan-handle PCR, which uses the known 5 ’end sequence and unknown 3’ end chaperone sequence to amplify the DNA at the breakpoint with a template that resembles a pan and a stem, is a novel approach to clone the cleavage site of the MLL gene, Can be extended to the unknown 3 ’end of the breakpoint cluster region.