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目的采用超高效液相色谱-四级杆-飞行时间串联质谱(UHPLC-Q-TOF/MS)法对红参醇提液中各成分进行分析和鉴定。方法色谱分离采用Poroshell 120 EC-C18色谱柱(3.0 mm×100 mm,2.7μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相梯度洗脱,流速为0.4 m L·min-1;采用双电喷雾离子源(Dual ESI),在负离子模式下采集数据;一级质谱提供精确的分子量信息,误差限制在5 ppm以内,得到该分子量最适的元素组成;二级质谱提供裂解碎片信息,与相关数据库和相关文献进行比对,推测其可能的化学结构。结果初步鉴定了红参醇提液中55个成分,包括原人参二醇型皂苷(PPD)27个,原人参三醇型皂苷(PPT)12个,齐墩果烷型皂苷1个,其他类成分15个。其中,10个化合物尚未有相关文献报道,结构需进一步确认。结论该研究丰富了对红参中化学物质的认识,为红参药效物质研究奠定了基础。
OBJECTIVE To analyze and identify the constituents of red ginseng ethanol extract by ultra performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF / MS) Methods Chromatographic separation was performed on a Poroshell 120 EC-C18 column (3.0 mm × 100 mm, 2.7 μm) using a mobile phase of 0.1% formic acid (A) -acetonitrile (B) as the mobile phase at a flow rate of 0.4 m L · min- 1; dual ESI, data acquisition in negative ion mode; MS provides accurate molecular weight information with an error limit of less than 5 ppm, resulting in an elemental composition with optimum molecular weight; MS provides MS Debris information, and related databases and related literature to compare, speculated that its possible chemical structure. Results The preliminary identification of 55 components of the red ginseng alcohol extract, including 27 of the original ginseng diol type saponin (PPD), 12 of the original ginseng saponin (PPT), oleanane type saponin 1, other types 15 ingredients. Among them, 10 compounds have not been reported in the literature, the structure needs further confirmation. Conclusion This study enriches the cognition of chemical substances in red ginseng and lays a foundation for the research on the pharmacodynamics of red ginseng.