论文部分内容阅读
目的 探讨端粒酶活性与P53、c-myc、bcl- 2基因在膀胱癌发生发展中的作用以及端粒酶活性与 3种与癌相关基因之间的关系。方法 采用端粒酶重复序列扩增法及酶联免疫吸附法和电脉法检测 58例膀胱癌、1 0例癌旁粘膜及 1 0例正常膀胱粘膜的端粒酶活性 ,采用免疫组织化学S -P法检测P53、c-myc、bcl- 2蛋白的表达。结果 膀胱癌组织端粒酶活性、P53、c -myc和bcl- 2蛋白阳性率分别为 86 .2 %、60 .3 %、68.9%和 81 % ,癌旁组织端粒酶活性、c -myc和bcl- 2蛋白阳性率为 1 0 %、1 0 %和 2 0 % ,P53蛋白为阴性 ,正常组织中端粒酶活性、P53、c-myc蛋白为阴性 ,bcl- 2蛋白阳性率为 1 0 % ,膀胱癌组织显著高于癌旁及正常组织 (P <0 .0 1 ) ,不同病理分级、临床分期之间端粒酶活性无显著性差异 (P >0 .0 5) ,P53、c -myc和bcl- 2异常表达有显著性差异 (P <0 .0 1或P <0 .0 5)。端粒酶活性与P53、c-myc、bcl- 2蛋白表达之间无明显相关性 (P >0 .0 5)。结论 端粒酶活化P53、c-myc和bcl- 2基因均参与膀胱癌的发生发展过程 ,联合检测有助于膀胱癌的早期诊断 ,端粒酶与P53、c-myc和bcl- 2无明显相关性 ,提示膀胱癌是由多基因参与的疾病
Objective To investigate the role of telomerase activity and P53, c-myc, bcl-2 gene in the development of bladder cancer and the relationship between telomerase activity and three kinds of cancer-related genes. Methods The telomerase activity of 58 cases of bladder cancer, 10 cases of paracancerous mucosa and 10 cases of normal bladder mucosa were detected by telomerase repeat amplification, enzyme-linked immunosorbent assay (ELISA) and electropuncture method. Immunohistochemistry S -P method to detect the expression of P53, c-myc, bcl-2 protein. Results The positive rates of telomerase activity, P53, c-myc and bcl-2 in bladder cancer tissues were 86.2%, 60.3%, 68.9% and 81%, respectively. The telomerase activity, c -myc The positive rates of bcl-2 and bcl-2 were 10%, 10% and 20%, respectively. The positive rate of bcl-2 protein in normal tissues was negative, P53 and c-myc were negative (P <0.01). There was no significant difference in telomerase activity between different pathological grades and clinical stage (P> 0.05), P53, c There was significant difference between -myc and bcl-2 (P <0.01 or P <0.05). There was no significant correlation between telomerase activity and P53, c-myc, bcl-2 protein expression (P> 0.05). Conclusion Telomerase activation of P53, c-myc and bcl-2 genes are involved in the development and progression of bladder cancer. Combined detection is helpful for the early diagnosis of bladder cancer. Telomerase was not associated with P53, c-myc and bcl-2 Correlation, suggesting that bladder cancer is a multi-gene involved in the disease