论文部分内容阅读
在间接免疫荧光技术(IFA)诊断寄生虫病试验中,细粒棘球蚴抗原的来源日益紧缺,而从实验动物啮齿类——泡状棘球蚴模型获取泡状棘球蚴抗原较方便。故本文作者用IFA以细粒棘球蚴和泡状棘球蚴的原头蚴抗原分别对同种和异种抗血清作比较研究,以探索同种抗原在改善泡状棘球蚴病的免疫诊断质量及其在血清流行病学调查中应用的可能性。所使用的泡状棘球蚴抗原材料取自实验性感染长爪沙鼠腹腔中的泡状棘球蚴,用胃蛋白酶溶液消化45分钟,从混悬液中可得到活的原头蚴。细粒棘球蚴抗原取自自然感染动物马的肝包虫囊液,经离心和洗涤后,获得
In the indirect immunofluorescence assay (IFA) for the diagnosis of parasitic diseases, the origin of Echinococcus granulosus antigen is increasingly scarce, whereas it is more convenient to obtain the Echinococcus epidermidis antigen from the experimental rodent-alveolar hydatid disease model. Therefore, the authors used IFA with Echinococcus granulosus and Echinococcus granulosus protoscolex antigens of the same and different anti-sera were compared to explore alloantigens in the improvement of immune response to bubble alveolar echinococcosis Quality and Its Potential for Serological Epidemiological Surveys. The somatic alveolar echinococcus antigen material used was taken from the bubbly hydatid cysts in the peritoneal cavity of experimental gerbils infected with pepsin and digested with pepsin for 45 minutes to obtain live protocereces from the suspension. Echinococcus granulosus antigen was obtained from hepatic hydatid cyst fluid naturally infected with animal horse, obtained after centrifugation and washing