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In order to clarify the damage mechanism of the subway structure, the dynamic soil-structure interaction and the dynamic forces acting on the structure, a series of shaking table tests and simulation analyses were performed. The seismic response of the structure and the dynamic forces acting on the structure due to sinusoidal and random waves were investigated with special attention to the dynamic soil-structure interaction. The result shows that the compression seismic soil pressures and extension seismic soil pressures simultaneously act on the sidewalls, and big shear stress also acts on the ceiling slab due to horizontal excitation. The seismic soil pressure could be approximated to hyperbola curve, and reached a peak value with increase of the shear strain of the model ground. In addition, a slide and exfoliation phenomenon between the structure and the surrounding ground was simulated, using the nonlinear analyses. The foundation is provided for amending the calculation method of seismic soil pressure and improving the anti-earthquake designing level of underground structure.