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前言几十年来,植物组织培养有了很大的发展,并广泛应用于花卉,园艺和农作物及其它领域中,特别是近年来,国内外对中草药也进行了研究。我们从青蒿头状花序诱导出植株,其无菌苗茎切段的再生植株93天现蕾,这将为中药青蒿的试管育种和定向培育提供了某些技术。材料和方法青蒿Artemisia apiacea Hance取自我所苗圃内,植株的发育时期为花蕾期,取其花枝,在室内将其花枝剪成单个花序。用70%酒精消毒30秒,用5%次氯酸钠消毒10
Preface For decades, plant tissue culture has been greatly developed, and is widely used in flowers, horticulture and crops and other fields, especially in recent years, Chinese and foreign herbalists have also been studied. We induced plants from A. annua and regenerated plants from stem segments of sterile seedlings to bud for 93 days. This provided some techniques for in vitro tube breeding and directional cultivation of A. annua. MATERIALS AND METHODS Artemisia apiacea Hance was taken from our nursery. The plants were in the budding stage of development. Their floral branches were cut and their flowering branches cut into single inflorescence indoors. Disinfect 30% with 70% alcohol and disinfect 10% with 5% sodium hypochlorite