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目的 用氨基比林和亚硝酸钠诱发叙利亚地鼠肝内胆管癌（ＩＣＣ）动物模型，观察胆管癌发生过程的病理变化。方法以 ８００ ｍｇ／Ｌ的氨基比林和亚硝酸钠溶液作为 ５０只 ６周龄叙利亚地鼠的饮用水，每周饮用 ６ ｄ，连续饮用 ２４周，定期分批处死动物，观察肝脏组织的病理学改变。结果 诱癌早期出现卵圆细胞增生、管状增生及小胆管的缀性增生，中期主要病变为肝内胆管上皮乳头状增生、化生、不典型增生和胆管纤维化，晚期可见肝内胆管腺瘤和腺癌。晚期肝内胆管癌的发生率达５０．０％（１０／２０）。结论该动物模型与人类肝内胆管癌发生发展的过程相似，方法简便、经济、可重复性好，具有良好的应用前景。肝内胆管的各种增生性病变与ＩＣＣ密切相关，尤其是不典型增生和胆管腺瘤，应视为ＩＣＣ的高危性癌前病变。
Objective To investigate the pathological changes of cholangiocarcinoma in Syrian Hamster induced intrahepatic cholangiocarcinoma (ICC) by using aminopyrine and sodium nitrite. METHODS: 800 mg/L aminopyrine and sodium nitrite solutions were used as drinking water for 50 six-week-old Syrian hamsters. They were consumed for 6 days each week for 24 weeks. Animals were killed in batches periodically to observe the liver tissue diseases. Science change. Results Oval cell hyperplasia, tubular hyperplasia, and conjunctival hyperplasia of small bile ducts appeared early in the induction of cancer. The main lesions in the middle stage were papillary hyperplasia, metaplasia, atypical hyperplasia, and bile duct fibrosis in the intrahepatic bile duct. Late intrahepatic bile duct adenomas were seen. And adenocarcinoma. The incidence of advanced intrahepatic cholangiocarcinoma was 50.0% (10/20). Conclusion This animal model is similar to the development of human intrahepatic cholangiocarcinoma. The method is simple, economical, and reproducible. It has a good application prospect. The various proliferative lesions of intrahepatic bile ducts are closely related to ICC, especially atypical hyperplasia and bile duct adenomas, which should be regarded as high risk precancerous lesions of ICC.