精于与卵细胞结合形成受精卵前需要获能和发生顶体反应。此时顶体内的各种酶(主要是顶体蛋白酶和透明质酸酶)释放出来,溶解放射冠和透明带,使精子能够穿入卵细胞,形成受精卵。因此,如果使用顶体酶抑制剂,通过抑制顶体反应而抑制酶的释放,就可抑制精卵结合,从而达到避孕的目的。我们运用国外已广泛使用的小鼠精卵细胞体外结合的实验对我所合成的顶体酶抑制剂4-胍基苯甲酸4-甲酸乙酯(EGB,4-Ethyl-phenyl,4-guanidinobenzoate)的作用进行了初步评价。 Expertise in combination with the formation of fertilized eggs and egg cells need to be able to take place and the acrosome reaction. At this point the various enzymes in the acrosome (mainly acrosin and hyaluronidase) are released, dissolving the corona and zona pellucida, allowing the sperm to penetrate into the egg cells to form fertilized eggs. Therefore, if the use of acrosin inhibitors, inhibition of acrosome inhibition by inhibition of enzyme release, you can inhibit sperm binding, so as to achieve the purpose of contraception. In this study, we used the in vitro binding assay of mouse oocyte, which has been widely used in foreign countries, to study the effect of 4-ethyl-4-guanidinobenzoate (EGB) The role of a preliminary evaluation.