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目的:说明在睾丸发育、禁食和实验性隐睾这样的生理和病理生理条件下,睾丸神经肽 Y(NPY)基因的分布和调控。方法:首先使用出生3天、2周、3周和6周的 C57BL/6J 雄性小鼠。然后分别将 C57BL/6J 成年雄性小鼠禁食46 h 和进行72天的实验隐睾处理。通过原位杂交组织化学法来检测 NPY 转录子。结果:所有的年龄组和实验条件下,NPY 转录子只在间质细胞中表达。NPY mRNA 水平随年龄显著增加(从初生到青春期前[P<0.01]以及从青春期前到青春期后[P<0.01])。48天的禁食导致下丘脑弓状核中的 NPYmRNA 水平显著增加(P<0.01),睾丸里却没有增加。72天的实验性隐睾不能调控 NPY 基因在睾丸中的表达。结论:NPY 基因表达分布在莱氏细胞中,并随睾丸的发育呈线性增加。睾丸的 NPY 表达调控与下匠脑的不同。
Objective: To describe the distribution and regulation of testosterone neuropeptide Y (NPY) gene in physiological and pathophysiological conditions such as testicular development, fasting and experimental cryptorchidism. Methods: C57BL / 6J male mice born at 3 days, 2 weeks, 3 weeks and 6 weeks were used. C57BL / 6J adult male mice were then each fasted for 46 h and subjected to experimental cryptorchidism for 72 days. NPY transcripts were detected by in situ hybridization histochemistry. RESULTS: NPY transcripts were expressed only in stromal cells under all age groups and experimental conditions. NPY mRNA levels increased significantly with age (from birth to pre-puberty [P <0.01] and from pre-puberty to adolescence [P <0.01]). 48-day fasting resulted in a significant increase in NPY mRNA levels in the arcuate nucleus of the hypothalamus (P <0.01) but not in the testes. 72 days of experimental cryptorchidism can not regulate the expression of NPY gene in testis. CONCLUSION: The NPY gene expression is distributed in Lai cells and shows a linear increase with testicular development. The regulation of NPY expression in testes is different from that in brains.