论文部分内容阅读
目的:探讨NDRG2在上皮性卵巢癌中的表达及其与预后的关系。方法:收集新鲜的上皮性卵巢癌和正常卵巢组织各15例,采用real-time PCR检测和比较其NDRG2 m RNA的表达。收集上皮性卵巢癌病理切片96例进行免疫组化检测其NDRG2蛋白的表达,并收集患者的临床病理资料,随访患者的生存情况,分析NDRG2蛋白的表达与上皮性卵巢癌患者临床病理特征和预后的关系。结果:上皮性卵巢癌组织中NDRG2 mRNA和蛋白的表达均显著低于正常卵巢组织(P<0.05)。NDRG2在上皮性卵巢癌组织中的表达随着上皮性卵巢癌病理分期的升高而降低,而且其表达降低和患者不良预后显著相关(P=0.002),但其表达和不同上皮性卵巢癌的病理类型、分化程度以及年龄均无明显相关性。去除手术病理分期的影响,NDRG2表达下调和肿瘤细胞减灭术的满意程度以及术后规范化疗是三个影响上皮性卵巢癌预后的重要因素。结论:NDRG2在上皮性卵巢癌中的表达下降,并与患者的不良预后呈显著正相关。
Objective: To investigate the expression of NDRG2 in epithelial ovarian cancer and its relationship with prognosis. Methods: Fresh epithelial ovarian cancer and normal ovarian tissue were collected in 15 cases. The expression of NDRG2 mRNA was detected and compared by real-time PCR. 96 cases of epithelial ovarian cancer were collected to detect the expression of NDRG2 protein by immunohistochemistry. The clinical and pathological data were collected and the survival of patients were followed up. The expression of NDRG2 protein and the clinicopathological characteristics and prognosis of epithelial ovarian cancer Relationship. Results: The expression of NDRG2 mRNA and protein in epithelial ovarian cancer was significantly lower than that in normal ovarian tissue (P <0.05). The expression of NDRG2 in epithelial ovarian cancer tissues decreased with the increase of the pathological stage of epithelial ovarian cancer, and the expression of NDRG2 was significantly correlated with the poor prognosis of patients (P = 0.002). However, the expression of NDRG2 was significantly different from that of epithelial ovarian cancer Pathological type, degree of differentiation and age were not significantly correlated. Removal of the pathological stage of surgery, NDRG2 expression downregulation and tumor cytoreductive surgery satisfaction and postoperative standard chemotherapy are three important prognostic factors in epithelial ovarian cancer. Conclusion: The expression of NDRG2 is down-regulated in epithelial ovarian cancer, which is positively correlated with the poor prognosis of patients.