论文部分内容阅读
目的探讨肾络通药物血清对大鼠系膜细胞外基质(ECM)不同成分的抑制作用及对转化生长因子β1(TGFβ1)表达的调节作用。方法以体外培养大鼠肾小球系膜细胞(MCs)及血清药理学方法为基础,采用酶联免疫吸附试验(ELAIS)检测细胞外基质中纤维连接蛋白(FN)、Ⅳ型胶原(ColⅣ)的含量;采用逆转录多聚酶链反应(RT PCR)检测TGFβ1mRNA的表达。结果细胞外基质FN、ColⅣ的含量与TGFβ1mRNA的表达呈正相关关系,肾络通对二者均有抑制作用。结论肾络通能够减少细胞外基质的积聚,抑制TGFβ1的分泌与表达,从而进一步明确其防治多种肾小球疾病,延缓肾小球硬化的作用机制。
Objective To investigate the inhibitory effect of Shenluotong drug serum on different components of rat mesangial extracellular matrix (ECM) and the regulation of transforming growth factor β1 (TGFβ1) expression. Methods Based on the in vitro culture of glomerular mesangial cells (MCs) and serum pharmacology, enzyme linked immunosorbent assay (ELAIS) was used to detect fibronectin (FN) and type IV collagen (ColIV) in extracellular matrix. The content of TGFβ1 mRNA was detected by reverse transcription polymerase chain reaction (RT PCR). Results There was a positive correlation between the content of extracellular matrix FN and Col IV and the expression of TGFβ1 mRNA. Shenluotong had inhibitory effects on both of them. Conclusion Shenluotong can reduce the accumulation of extracellular matrix and inhibit the secretion and expression of TGFβ1, thus further clarifying the mechanism of preventing and treating various glomerular diseases and delaying glomerulosclerosis.