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目的:研究川芎嗪的抗抑郁作用及其对HPA轴的影响。方法:C57小鼠随机分为对照组、川芎嗪低剂量组(120 mg/kg,po)、川芎嗪高剂量组(240 mg/kg,po)、阿米替林组(5 mg/kg,po),在小鼠强迫游泳及悬尾实验抑郁模型上,观察药物对小鼠强迫游泳不动时间及悬尾不动时间的影响;放射免疫法测定小鼠血清促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)及皮质酮(CORT)含量。结果:与空白对照组比较,川芎嗪120、240mg/kg均能显著减少小鼠悬尾和强迫游泳的不动时间;HPA轴激素水平检测结果显示,川芎嗪120、240 mg/kg可逆转应激造成的血清CRH、ACTH及CORT含量升高。结论:川芎嗪具有显著的抗抑郁效果,对神经内分泌系统的调节可能是其发挥抗抑郁作用的机理之一。
Objective: To study the antidepressant effect of ligustrazine and its effect on HPA axis. Methods: C57 mice were randomly divided into control group, Ligustrazine low dose group (120 mg / kg po), Ligustrazine high dose group (240 mg / kg po), Amitriptyline (5 mg / po) were used to observe the effects of drugs on immobility time and immobility time of forced swimming in mouse forced swimming and tail-hanging experiment depression model. Radioimmunoassay was used to detect the levels of serum corticotropin-releasing hormone (CRH) ), Adrenocorticotropic hormone (ACTH) and corticosterone (CORT) content. Results: Compared with the blank control group, tetramethylpyrazine 120 and 240 mg / kg significantly reduced immobility and forced swimming time. HPA axis hormone test results showed that tetramethylpyrazine 120 and 240 mg / kg were reversible Serum into the serum CRH, ACTH and CORT content increased. CONCLUSION: Tetramethylpyrazine has significant antidepressant effect and regulation of neuroendocrine system may be one of the mechanisms of its antidepressant effect.