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应用ＢＮＹＶＶ抗体球蛋白，建立了快速检测ＢＮＹＶＶ的间接酶跌免疫吸附技术（Ｉｎｄｉｒｅｃｔ－ＥＬＬＳＡ），该法检测含抗原材料仅需４．５小时，较国际通用程序缩短２０余小时。主要程序包括：抗原经３７℃包被２小时后，加ＢＮＹＶＶ抗体球蛋白ＩｇＧ，３７℃孵育１小时，然后加过氧化物酶标记的单抗兔ＩｇＧ，３７℃孵育１小时，最后加底物液邻笨二胺，常温下反应１５～３０分钟，应用该法对采集的４份标样进行测定，其中两份测出含有ＢＮＲＶＶ，对不同抗感丛根病性品种（系）经室内病土盆栽或病田栽培，调查病情并取样测定根部ＢＮＹＶＶ含量，结果表明。品种间存在显著差异，ＢＮＹＶＶ含量、病情与品种（系）抗病性表现基本一致。
By using BNYVV antibody globulin, Indirect-ELLSA was established to detect BNYVV rapidly. The assay of antigen-containing material only took 4.5 hours, which was more than 20 hours shorter than the international standard procedure. The main program includes: After the antigen is coated for 2 hours at 37 ° C, add the BNYVV antibody globulin IgG and incubate for 1 hour at 37 ° C, then add the peroxidase-labeled monoclonal IgG rabbit and incubate for 1 hour at 37 ° C, add the substrate Liquid orally stupid diamine, the reaction at room temperature for 15 to 30 minutes, the application of the method of the four samples collected were measured, of which two were measured containing BNRVV, resistance to different resistance Rhizotomy strains (lines) by indoor disease Potted plants or disease cultivation, investigation of the disease and sampling determination of root BNYVV content, the results show. There were significant differences among varieties. BNYVV content, disease status and varieties (lines) showed basically the same disease resistance.