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对两种杂交石斑鱼子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)的线粒体DNA细胞色素氧化酶Ⅰ(COⅠ)基因序列进行了测序分析。在15个样本中,同源序列片段(1 551bp)中共检测到9个单倍型和249个核苷酸多态位点。序列差异分析和遗传距离比较结果显示,核苷酸序列同源性在88.1%～100%之间,无明显遗传分化。虎龙斑的2个COⅠ基因单倍型与母本棕点石斑鱼单倍型的同源性为99%和100%,而与父本鞍带石斑鱼的同源性均为88.1%。青龙斑的2个单倍型与母本斜带石斑鱼的3个单倍型的同源性在99.7%～100%之间,而与父本鞍带石斑鱼的同源性分别为89.3%和89.4%,结果表明两种杂交子一代在线粒体DNA COⅠ基因上严格遵循母性遗传规律。
The mitochondrial DNA cytochrome oxidase Ⅰ (COⅠ) gene of two crossbred grouper (blue dragonfly and tiger dragonfly) and their parents (slash grouper, brown grouper and saddle grouper) Sequences were sequenced. In 15 samples, 9 haplotypes and 249 nucleotide polymorphisms were detected in homologous sequence fragments (1 551 bp). Sequence difference analysis and genetic distance comparison showed that the nucleotide sequence homology between 88.1% to 100%, no significant genetic differentiation. Homology of the two COI haplotypes in Tiger’s locust was 99% and 100% identical to haplotypes of the brown spotted grouper and 88.1% . The homology of three haplotypes between two haplotypes of Qinglong spot and that of female parental grouper was between 99.7% and 100%, while their homology with the grouper of the parental saddleband was 89.3% and 89.4% respectively. The results showed that the two hybrids strictly followed the maternal genetic rule in mitochondrial DNA COⅠgene.