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目的:研究中药补肾助孕汤对人子宫内膜基质细胞体外培养影响。方法:将中药方剂补肾助孕汤制成培养基,体外培养子宫内膜基质细胞,观察其成长状况及白血病抑制因子(leukaemia inhibitory factor,LIF)、表皮生长因子(epidermalgrowth factor,EGF)、降钙素(calcitionin,CT)的表达。结果:与对照组相比,补肾助孕汤可以促进子宫内膜细胞在体外增殖,免疫组化显示LIF(192.903 1±14.534 2 vs 183.156 8±9.189 1,P<0.001,t=5.35)、EGF(196.479 8±2.907 1 vs 184.506 4±3.733 5,P<0.001,t=26.1)、CT(183.185 6±8.422 7 vs 176.418 4±0.448 0,P<0.005,t=7.45)因子在子宫内膜基质细胞的表达相对于对照组增强。结论:中药方剂补肾助孕汤可以促进子宫内膜细胞在体外增殖,增强LIF、EGF、CT等因子的表达。
Objective: To study the effect of Bushen Zhuyun Decoction on the culture of human endometrial stromal cells in vitro. Methods: The recipe of Bushen Zhuyun Decoction was made into medium and the endometrial stromal cells were cultured in vitro. The growth status, leukemia inhibitory factor (LIF), epidermal growth factor (EGF) Expression of calcitionin (CT). Results: Compared with the control group, Bushen Zhuyun Decoction could promote the proliferation of endometrial cells in vitro. Immunohistochemistry showed LIF (192.903 ± 14.534 2 vs 183.156 8 ± 9.189 1, P <0.001, t = 5.35), EGF (196.479 8 ± 2.907 1 vs 184.506 4 ± 3.733 5, P <0.001, t = 26.1), CT (183.185 6 ± 8.422 7 vs 176.418 4 ± 0.448 0, P <0.005, t = 7.45) The expression of cells is increased relative to the control group. Conclusion: Bushen Zhuyun Decoction can promote the proliferation of endometrial cells in vitro and enhance the expression of LIF, EGF, CT and other factors.