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目的评估荧光原位杂交(fluorescence in situ hybridization,FISH)技术在快速检测流产物染色体数目异常的价值。方法对514例流产物应用13、16、18、21、22、X、Y染色体特异性DNA探针进行FISH技术检测。结果 513份组织标本检测成功,1份标本无荧光信号,共检出染色体数目异常141例,其中各种常染色体三体型60例,性染色体异常37例,三倍体32例,四倍体10例,染色单体2例。结论应用FISH技术检测流产物染色体数目异常,简单、快速并且准确,可以进行流产病因分析并且为再次妊娠风险评估提供依据。
Objective To evaluate the value of fluorescence in situ hybridization (FISH) in the rapid detection of chromosomal abnormalities in stream products. Methods 514 cases of abortion were detected by FISH using 13, 16, 18, 21, 22, X and Y chromosome specific DNA probes. Results Totally 513 tissue samples were detected successfully. One sample showed no fluorescence signal, and 141 abnormal chromosomes were detected. Among them, there were 60 cases with various trisomy, 37 cases with sex chromosome abnormality, 32 cases with triploid and 10 cases with tetraploid 10 Cases, 2 cases of chromatids. Conclusion FISH is a simple, rapid and accurate method to detect the number of chromosomes in abortion. It can be used to analyze the cause of abortion and provide the basis for the risk assessment of re-pregnancy.