论文部分内容阅读
巴西固氮螺菌(Azospirillum brasilense)可与多种重要作物联合固氮,具有作为生物肥料的潜能.固氮基因(nif)的转录激活蛋白NifA和PⅡ信号转导蛋白家族成员GlnB是参与该菌固氮调控的两个关键蛋白,且NifA的活性调控依赖于GlnB.研究结果表明,在无氮条件下GlnB与NifA蛋白N端结构域存在相互作用,且N端18和53位酪氨酸突变为苯丙氨酸的NifA与GlnB的互作增强.研究还发现,NifA蛋白N端66~88位和165~176位氨基酸区域对于与GlnB蛋白的相互作用是必需的.
Azospirillum brasilense can be combined with a variety of important crops to fix nitrogen, which has the potential of being used as a bio-fertilizer. NifA, a transcriptional activator of nitrogenase (NIF), and GlnB, a member of PII signal transduction protein family, Two key proteins, and the regulation of NifA activity depends on Gln B. The results show that there is interaction between GlnB and NifA N-terminal domain in the absence of nitrogen, and N-terminal 18 and 53 tyrosine mutations to amphetamine The interaction of acid NifA and GlnB was enhanced.The study also found that amino acids 66-88 and 165-176 of the N-terminus of NifA protein are necessary for the interaction with GlnB protein.