为了确诊山东省某些鸡场送检的疑似心包积液综合征病例,取病死鸡肝脏进行研磨,经鸡胚绒毛膜尿囊腔途径接种10日龄SPF鸡胚,成功获得1株病毒,并进行了鸡胚病变特征观察、PCR检测、基因测序、BLAST分析及动物回归试验。结果表明:成功分离出的1株病毒为鸡心包积液-肝坏死综合征病毒;经基因序列测定表明该病毒是禽腺病毒Ⅰ亚群C种血清型4型(FAV-4)。 In order to confirm the cases of suspected pericardial effusion syndrome in some chicken farms in Shandong Province, the liver of the dead chickens was grinded and 10-day-old SPF chick embryos were inoculated through chick chorioallantoic cavity, and 1 virus was successfully obtained Chicken embryo lesions were observed, PCR detection, gene sequencing, BLAST analysis and animal regression test. The results showed that one of the viruses successfully isolated was chicken pericardial effusion-liver necrosis syndrome virus. The gene sequence showed that the virus was subtype C of avian adenovirus subtype C (4) (FAV-4).