论文部分内容阅读
目的 了解鼠疫菌102kb pgm基因座一端IS100的缺失与pgm~1稳定性的关系。方法 采用聚合酶链式反应(PCR)扩增国内各型鼠疫菌102kb pgm基因座色素沉着这一端IS100的片段,并选择克隆测序分析。结果 通过PCR扩增,其中只能扩增出约2560bp左右条带的生态型,也就是其102kb pgm基因座色素沉着这一端IS100的片段缺失,除锡林郭勒高原布氏田鼠型,还有北天山东段型、北天山西段A、B型,其pgm~+表型非常稳定;而有一部分菌株的扩增结果为阴性另一部分菌株扩增出4492bp条带的生态型,扩增出4492bp条带的菌株102kb pgm基因座色素沉着这一端有IS100,结果为阴性的菌株其整个102kb pgm基因座可能已经丢失,此种情况包括祁连山型,青藏高原型,冈底斯山型,滇西纵谷型等,其pgm~+表型表现出不稳定。结论 鼠疫菌102kb pgm基因座一侧缺失IS100的菌株,可具有较稳定的pgm~+表型,而鼠疫菌102kb pgm基因座两侧有IS100的菌株,pgm~+表型不稳定。
Objective To understand the relationship between the deletion of IS100 at one end of 102kb pgm locus and the stability of pgm ~ 1. Methods PCR amplification was used to amplify the fragment of IS100 at the end of pigmentation of 102kb pgm locus of Y. pestis in China. The cloned sequence analysis was carried out. The results of PCR amplification, which can only be amplified about 2560bp band around the ecological type, that is, its 102kb pgm locus pigmentation IS100 end of this fragment is missing, in addition to Xilin Gol plateau fieldbuster type, as well as North Tianshan In the western section of North Tianshan Mountains, the pgm ~ + phenotype was very stable. However, some strains showed negative amplification results and the other strains amplified 4492bp ecotypes and amplified 4492bp bands Strain 102kb pgm Locus Pigmentation of this strain with IS100, negative result, may have lost its entire 102kb pgm locus, including the Qilian Mountains, Tibetan Plateau, Gangdese, West Yunnan Rift Etc., its pgm ~ + phenotype showed instability. Conclusion The strains with IS100 deletion on the side of the 102kb pgm locus of P. yunnanensis have a stable pgm + phenotype. However, strains of strain IS100 on both sides of the 102kb pgm locus in P. infestans strain are instable.