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目的建立HPLC法同时测定疏通散中有效成分三七皂苷R1、人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb1的含量。方法采用HPLC法,色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%(φ)磷酸水溶液,梯度洗脱,流速为1.0 m L·min~(-1),检测波长为203 nm,柱温为30℃。结果三七皂苷R_1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb_1的质量浓度分别在0.115~0.575、0.230~1.150、0.090~0.450、0.155~0.775 g·L~(-1)内与峰面积呈良好的线性关系,相关系数分别为0.999 5、0.999 3、0.999 2、0.999 1,平均回收率分别为99.1%﹑99.1%﹑98.9%、98.6%,RSD分别为1.3%、1.2%、0.9%、1.2%。结论本方法可作为疏通散的质量控制方法之一。
OBJECTIVE To establish an HPLC method for the simultaneous determination of notoginsenoside R1, ginsenoside Rg_1, ginsenoside Re and ginsenoside Rb1 in Shu Tong San. Methods HPLC was performed on a Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile - 0.1% (φ) phosphoric acid and a gradient elution at a flow rate of 1.0 m L · min -1. , The detection wavelength was 203 nm, the column temperature was 30 ℃. Results The mass concentrations of notoginsenoside R_1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb_1 in the range of 0.115-0.575, 0.230-1.150, 0.090-0.450, 0.155-0.775 g · L -1 were respectively The correlation coefficients were 0.999 5,0.999 3,0.999 2,0.999 1 respectively. The average recoveries were 99.1%, 99.1%, 98.9% and 98.6%, respectively, and the RSDs were 1.3%, 1.2% and 0.9% respectively, 1.2%. Conclusion This method can be used as one of the quality control methods to disperse bulk.