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目的探讨β-抑制蛋白2与Toll样受体2(TLR2)在子宫内膜癌中的表达水平及临床意义和二者的关系。方法以滨州市人民医院2010年1月-2015年2月收集的60例组织标本为研究对象,其中30例为子宫内膜癌(包括复发转移癌15例),15例为子宫内膜非典型增生,15例为正常子宫内膜。采用免疫组化染色法检测β-抑制蛋白2及TLR2的表达水平。结果β-抑制蛋白2及TLR2在子宫内膜癌组织中的表达水平均显著高于非典型增生组织及正常内膜组织(P<0.01);β-抑制蛋白2与TLR2的表达水平呈正相关性(r=0.866,P<0.01)。TLR2、核转录因子kappa B(NF-κB)及髓样分化因子88(MYD88)的表达水平在β-抑制蛋白2-RNAi干扰组显著下降(P<0.05),在全长质粒过表达组中的表达水平显著增高(P<0.05)。结论β-抑制蛋白2及TLR2的高表达作用于子宫内膜癌的发生、发展及转移,且二者关系密切,互相作用。β-抑制蛋白2可成为TLR2的激动剂,激活相关途径的MYD88依赖型信号转导通路,进而实现NF-κB活化。
Objective To investigate the expression and clinical significance of β-arrestin 2 and Toll-like receptor 2 (TLR2) in endometrial carcinoma and their relationship. Methods Totally 60 cases of tissues collected from Binzhou People’s Hospital from January 2010 to February 2015 were selected as the research objects, of which 30 cases were endometrial carcinoma (including 15 cases with recurrent and metastatic carcinoma) and 15 cases were endometrial atypical Hyperplasia, 15 cases of normal endometrium. Immunohistochemical staining was used to detect the expression of β-arrestin 2 and TLR2. Results The expressions of β-arrestin 2 and TLR2 in endometrial carcinoma tissues were significantly higher than those in atypical hyperplasia tissues and normal endometrial tissues (P <0.01). The expression of β-arrestin 2 and TLR2 was positively correlated (r = 0.866, P <0.01). The expression levels of TLR2, NF-κB and MYD88 were significantly decreased in the β-arrestin 2-RNAi interference group (P <0.05). In the full-length plasmid overexpression group The expression level was significantly higher (P <0.05). Conclusion The high expression of β-arrestin 2 and TLR2 plays an important role in the development, progression and metastasis of endometrial carcinoma, and they are closely related and interact with each other. β-arrestin 2 can act as an agonist of TLR2 and activate the MYD88-dependent signal transduction pathway of related pathways, thereby activating NF-κB.