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本实验应用间接血凝试验(IHT)和酶联免疫吸附试验(ELISA),测定了20只输精管结扎后16—22个月的兔和24只对照家兔血清的抗精子抗体。IHT 检测结果是,对照组全为阴性,结扎组阳性率为100%,最早检出抗体时间在术后第3个月,抗体滴度范围为1:5—1:2560;ELISA 检测结果是,对照组阳性率为25%,光密度范围为0.0145—0.5180,结扎组阳性率为100%,最早检出抗体时间在术后第2个月,光密度范围为0.1230—1.1930比较两种试验结果发现,两者有显著的一致性。
In this study, indirect hemagglutination test (IHT) and enzyme-linked immunosorbent assay (ELISA) were used to determine the anti-sperm antibodies in sera from 16-22 months after vasectomy and 24 control rabbits. IHT test results were negative in the control group, the positive rate of ligation group was 100%, the first detection of antibody in the first 3 months after surgery, antibody titer range 1: 5-1: 2560; ELISA test results, The positive rate of control group was 25%, the optical density range was 0.0145-0.5180, the positive rate of ligation group was 100%, the earliest detection antibody time was in the second month after operation, the optical density range was 0.1230-1.1930. , There is a significant agreement between the two.