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目的:观察基质细胞衍生因子1α(stromal cell derived factor-1 alpha,SDF-1α)对体外培养的海马神经元突起延伸与凋亡的影响。方法:应用免疫细胞荧光方法观察SDF-1α和CXCR4在细胞的定位;以不同浓度的SDF-1α处理细胞,观察细胞形态的变化;并采用阻断剂AMD3100阻断CXCR4受体,观察其对细胞的影响;通过流式细胞仪检测细胞凋亡情况。结果:免疫荧光细胞化学方法显示SDF-1α和CXCR4主要表达在体外培养的海马神经元的胞膜和胞浆;不同浓度的SDF-1α改变了细胞的形态,促进了突起延伸;AMD3100则抑制了细胞突起增长;流式细胞仪检测显示SDF-1α减少了细胞凋亡。结论:SDF-1α可影响海马神经元突起延伸和凋亡效应,该机制可能与受体CXCR4有关。
Objective: To observe the effects of stromal cell derived factor-1 alpha (SDF-1α) on neurite outgrowth and apoptosis in cultured hippocampal neurons. Methods: Immunofluorescent staining was used to observe the cell localization of SDF-1α and CXCR4. SDF-1α cells were treated with different concentrations of SDF-1α to observe the changes of cell morphology. Blocking agent CXCR4 was used to block CXCR4 receptor, The effect of apoptosis was detected by flow cytometry. Results: Immunofluorescence cytochemistry showed that SDF-1α and CXCR4 were mainly expressed in the membrane and cytoplasm of hippocampal neurons cultured in vitro; different concentrations of SDF-1α changed the morphology of cells and promoted the extension of neurites; AMD3100 inhibited Cell protrusion increased; flow cytometry showed SDF-1α reduced apoptosis. Conclusion: SDF-1α can affect the neurite outgrowth and apoptosis in hippocampus, which may be related to the receptor CXCR4.