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目的:研究金鸡脚Phymatopteris hastate的化学成分及其抗氧化活性。方法:综合运用硅胶柱色谱、凝胶柱色谱、HPLC制备色谱等多种色谱法分离纯化金鸡脚中的化学成分;采用NMR等波谱学方法鉴定化合物结构;进一步通过DPPH法评价各化合物的抗氧化活性。结果:从金鸡脚乙酸乙酯层和正丁醇层中分离得到14个化合物,分别为4-O-β-D-吡喃葡萄糖基咖啡酸乙酯(1),山柰酚-7-O-α-L-吡喃鼠李糖苷(2),山柰酚-3,7-O-α-L-二吡喃鼠李糖苷(3),山柰酚-3-O-α-L-呋喃阿拉伯糖-7-O-α-L-吡喃鼠李糖苷(4),核桃苷(5),柚皮苷(6),柚皮素-7-O-β-D-吡喃葡萄糖苷(7),反式咖啡酸(8),反式咖啡酸-4-O-β-D-吡喃葡萄糖苷(9),4-O-β-D-吡喃葡萄糖基反式肉桂酸(10),2-O-β-D-吡喃葡萄糖基反式肉桂酸(11),2-O-β-D-吡喃葡萄糖基顺式肉桂酸(12),绿原酸乙酯(13),原儿茶酸(14)。抗氧化实验结果表明:化合物1～9,13,14显示了明确的抗氧化活性。结论:所有化合物均为首次从该属中分离得到;其中化合物1未见文献报道,推测可能为化合物9与乙醇反应后的人工产物;化合物1～9,13,14显示了明确的抗氧化活性。
Objective: To study the chemical constituents and antioxidative activity of Phymatopteris hastate. METHODS: The chemical constituents in the feet of Jinji were isolated and purified by silica gel column chromatography, gel column chromatography and HPLC-preparative chromatography. The chemical structures of the compounds were identified by NMR and other spectroscopic methods. The antioxidant activity of each compound was evaluated by DPPH method active. Results: Fourteen compounds were isolated from the layer of ethyl acetate and n-butanol, which were 4-O-β-D-glucopyranosyl caffeate (1), kaempferol- α-L-rhamnopyranoside (2), kaempferol-3,7-O-α-L-dipyridyl rhamnoside (3), and kaempferol-3-O-α-L- 7-O-β-D-glucopyranoside (4), walnoside (5), naringin (6), naringenin- 7), trans-caffeic acid (8), trans-caffeic acid -4-O-β-D-glucopyranoside (9), 4-O-β-D-glucopyranosyl transcinnamate ), 2-O-β-D-glucopyranosyl transcinnamic acid (11), 2-O-β-D-glucopyranosyl cis- , Protocatechuic acid (14). Antioxidant results showed that compounds 1 to 9, 13 and 14 showed definite anti-oxidative activity. CONCLUSION: All the compounds were isolated from this genus for the first time. Among them, Compound 1 was not reported in the literature and it was presumed that it could be an artificial product after the reaction of Compound 9 with ethanol. Compounds 1 to 9, 13 and 14 showed definite antioxidant activity .