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目的:探索中药纳达合剂对胆汁反流性胃炎(BRG)大鼠胃黏膜及相关炎症因子的影响。方法:采用灌胃牛胆酸钠+胰酶+卵磷脂法制备胆汁反流性胃炎模型。正常组、模型组灌胃生理盐水,纳达合剂低、中、高剂量组及麦滋林组分别予灌胃相应药物干预。实验结束,通过病理切片HE染色观察各组大鼠胃黏膜炎症变化情况,ELISA法检测大鼠胃黏膜白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(ICAM-1),Western blot方法检测大鼠胃黏膜单核细胞趋化因子-1(MCP-1)。结果:模型组大鼠胃黏膜炎症细胞浸润明显,炎症积分较正常组显著增高(P<0.01);与模型组比较,各药物干预组均可以显著改善大鼠胃黏膜炎症情况(P<0.01),其中纳达合剂组呈剂量依赖性,且与麦滋林组比较差异无统计学意义(P>0.05)。模型组大鼠胃黏膜炎症因子TNF-α、IL-8、ICAM、MCP-1蛋白表达较正常组显著增高(P<0.01);与模型组比较,纳达合剂各剂量组呈剂量依赖性地显著降低TNF-α、IL-8、ICAM、MCP-1蛋白表达(P<0.05或P<0.01),药麦滋林组亦可显著减少胃黏膜TNF-α、IL-8、ICAM、MCP-1蛋白表达(P<0.01),而纳达中、高剂量组在改善TNF-α表达方面优于麦滋林组(P<0.05或P<0.01),纳达高剂量组在改善ICAM-1表达方面优于麦滋林组(P<0.01)。结论:纳达合剂可显著改善胆汁反流性胃炎大鼠的胃黏膜病理,并对相关炎症因子有明显的抑制作用。
Objective: To explore the effect of traditional Chinese medicine Nada Mixture on gastric mucosa and related inflammatory factors in rats with bile reflux gastritis (BRG). Methods: Bile reflux gastritis model was prepared by intragastric administration of sodium chlorate, trypsin and lecithin. In the normal group, the rats in the model group were given gavage with normal saline, and the nadal mixture in the low, medium and high dose groups and the Maizhaolin group were given the corresponding drug interventions. At the end of the experiment, the histopathological changes of gastric mucosal inflammation were observed by HE staining. The levels of interleukin-8, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule 1 (ICAM-1), and Western blot was used to detect the expression of monocyte chemoattractant protein-1 (MCP-1) in rat gastric mucosa. Results: The infiltration of inflammatory cells in gastric mucosa of rats in model group was significant and the inflammation score was significantly higher than that in normal rats (P <0.01). Compared with model group, the intervention group of each drug could significantly improve gastric mucosal inflammation (P <0.01) , In which nadalix group was dose-dependent, and there was no significant difference between them (P> 0.05). Compared with the model group, the expression of TNF-α, IL-8, ICAM and MCP-1 in gastric mucosa significantly increased in model group than in normal group (P <0.01) The levels of TNF-α, IL-8, ICAM and MCP-1 in gastric mucosa were significantly decreased (P <0.05 or P <0.01) (P <0.01). However, middle and high dosage of Nadal was superior to Mazazin in improving the expression of TNF-α (P <0.05 or P <0.01), and high dosage of Nada in improving ICAM-1 The expression was superior to the Mazinger group (P <0.01). Conclusion: Nada Mixture can significantly improve gastric mucosal pathology in rats with bile reflux gastritis and significantly inhibit the related inflammatory cytokines.