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目的利用图像分析技术定量研究肺腺癌中程序性死亡配体1(programmed cell deathligand 1,PD-L1)的蛋白表达及其与临床病理参数之间的相关性,并对比PD-L1免疫组化彩色图像定量分析结果与人工判读结果的一致性。方法采用免疫组化En Vision两步法检测109例肺腺癌标本的PD-L1蛋白表达。从每张切片的肿瘤区域随机抽取5个视野进行图像采集及分析,计算每个病例的染色程度比值[(棕色区域面密度÷蓝色区域面密度)×棕色平均光密度],将该值≥0.05的病例定义为PD-L1蛋白表达图像分析结果阳性。人工判读结果系参照文献通用的阳性强度与阳性细胞百分数的乘积;实验结果均使用SAS 9.1软件分析。结果 PD-L1的染色程度比值(定量)在不同性别中具有明显差异性(P<0.05);PD-L1蛋白表达的定性结果与肿瘤的组织学分型密切相关(P<0.05);PD-L1免疫组化图像分析的定性结果与人工判读结果的一致率为88.073%(K=0.7157)。结论对肺腺癌PD-L1蛋白表达程度采用图像分析定量检测所获结果与人工判读法结果一致性较好,但前者更具有客观性和准确性。
Objective To quantitatively study the protein expression of programmed cell death ligand 1 (PD-L1) in lung adenocarcinoma and its correlation with the clinicopathological parameters by using image analysis technique. Compared with PD-L1 immunohistochemistry Consistency of Color Image Quantitative Analysis with Manual Interpretation Results. Methods Immunohistochemistry En Vision two-step method was used to detect the expression of PD-L1 protein in 109 cases of lung adenocarcinoma. Five fields were randomly selected from the tumor area of each section for image acquisition and analysis, and the ratio of staining degree (brown area density ÷ blue area density × brown average density) was calculated for each case, and the value ≧ A case definition of 0.05 was positive for PD-L1 protein expression image analysis. The result of manual interpretation is the product of the positive intensity and the percentage of positive cells that are common to the reference literature. The experimental results are all analyzed by SAS 9.1 software. Results PD-L1 staining was significantly different in different sex (P <0.05). The qualitative results of PD-L1 protein expression were closely related to histological type (P <0.05) The concordance rate between the qualitative and immunohistochemical images was 88.073% (K = 0.7157). Conclusion The results of quantitative analysis of PD-L1 protein in lung adenocarcinoma using image analysis are in good agreement with the results of manual interpretation, but the former is more objective and accurate.