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【目的】利用26S rDNA D1-D3区序列差异鉴别鸡血藤及其混淆品。【方法】使用改良十六烷基三甲基溴化铵(CTAB)法提取鸡血藤及其混淆品的总DNA,选择浓度较高的样品作为模板DNA,对26S rDNA D1-D3区进行PCR扩增、测序和序列比对分析。【结果】获得26S rDNA D1-D3区长约700 bp的序列。比对结果显示,不同产地之间鸡血藤的序列一致率为96%和100%,混淆品与正品比较的序列一致率为80%～96%。在26S rDNA D1-D3区384～472 bp的位置有足够的碱基差异位点用以区分鸡血藤及其混淆品。【结论】26S rDNA D1-D3区序列可以应用于鉴别鸡血藤及其混淆品。
【Objective】 The identification of Millettia and its adulterants was done by using the sequence differences of 26S rDNA D1-D3. 【Method】 The total DNA of Millettia and its mixture was extracted by modified cetyltrimethylammonium bromide (CTAB) method. The samples with higher concentration were selected as the template DNA, and PCR was performed on the 26S rDNA D1-D3 Amplification, sequencing and sequence alignment analysis. 【Result】 The sequence of 26S rDNA D1-D3 was about 700 bp in length. The results of comparison showed that the consistency rates of Millettia species from different regions were 96% and 100%, and the identical sequences of the confused and authentic samples were 80% ~ 96%. In the 26S rDNA D1-D3 area 384 ~ 472 bp position has enough base difference sites to distinguish Millettia and its confusion. 【Conclusion】 The 26S rDNA D1-D3 region sequence can be used to identify Millettia and its adulterants.