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目的观察两种不同粒细胞集落刺激因子(G CSF)动员剂(分泌型与包涵体型)对急性心肌梗死(AMI)患者骨髓血干细胞动员效率。方法一组予以包涵体型G CSF(商品名惠尔血)300μg(包涵体型G CSF组),每日2次,皮下注射,连续5天;另一组予以分泌型G CSF(商品名金磊赛强)300μg(分泌型G CSF组),每日2次,皮下注射,连续5天。第6日经美国Baxter公司生产的CS3000PLUS血细胞分离机,分离外周血干细胞,采集外周血干细胞悬液经流式细胞仪测定CD34+的细胞数量。结果给G CSF前及给G CSF后第3、4、5、6天两组间外周血中细胞表面标记蛋白CD34+细胞数量和白细胞计数无明显统计学差异;在应用两组不同的动员剂后,外周血中白细胞计数与动员时间变化曲线显示曲线高峰在动员后第5天;在包涵体型G CSF组,CD34+细胞数量与时间变化曲线高峰为第5天,但在分泌型G CSF组,CD34+细胞数量与时间变化曲线显示CD34+细胞数量在3～4天内呈急剧升高趋势,但在第5天后升幅明显减缓;显示分泌型G CSF组动员后外周血中干细胞下降较慢;患者外周血中CD34+细胞数量与白细胞计数变化呈正相关(r=0.835),与性别、体重、年龄及AMI发生时间无显著性相关。结论在AMI患者中应用两种不同G CSF动员剂,两组在外周血干细胞动员效率方面无明显统计学差异。
Objective To observe the mobilization efficiency of bone marrow stem cells in patients with acute myocardial infarction (AMI) by using two different mobilizing agents of granulocyte colony stimulating factor (G CSF). Methods One group was given inclusion body of G CSF (tradename Huier blood) 300μg (inclusion body type G CSF group), twice a day, subcutaneously for 5 consecutive days; the other group was secreted G CSF (trade name Jin Lei Saiqiang ) 300 μg (secreted G CSF group) twice a day, subcutaneously for 5 consecutive days. The sixth day by the United States CS3000PLUS Baxter blood cell separator, peripheral blood stem cells were isolated from peripheral blood stem cell suspension was collected to determine the number of CD34 + cells by flow cytometry. Results There was no significant difference in the number of peripheral blood CD34 + cells and white blood cell count between the two groups on the 3rd, 4th, 5th and 6th days before G CSF administration and after G CSF administration. After using two different mobilization agents , The curve of white blood cell count and mobilization time in the peripheral blood showed the peak curve at 5 days after mobilization. In the G CSF group, the number of CD34 + cells and the time curve peaked on the 5th day, but in the secretory G CSF group, CD34 + The curve of cell number and time showed that the number of CD34 + cells increased sharply within 3 ~ 4 days, but obviously increased slowly after 5 days. The number of CD34 + cells in peripheral blood decreased slowly after the mobilization of secretory G CSF group There was a positive correlation between the number of CD34 + cells and the change of white blood cell count (r = 0.835), but not with the gender, body weight, age and time of AMI. Conclusions Two different G CSF mobilizers were used in patients with AMI. There was no significant difference in the mobilization efficiency of peripheral blood stem cells between the two groups.