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目的 :在证明日本血吸虫重组蛋白酶B核酸疫苗Sj31BIN具有抗生殖免疫作用的基础上 ,联合使用IL 12 ,观察IL 12是否具有佐剂效果。方法 :分别用Sj31BIN +IL 12和IL 12免疫Balb/C小鼠。攻击感染后 6周计数成虫负荷和组织内虫卵数及肝脏表面虫卵结节数。结果 :Sj31BIN +IL 12免疫小鼠可降低成虫发育率。肝组织减卵率为5 9 74% ;肠组织减卵率为 5 9 6 0 % ;肝脏表面虫卵结节减少率为 71 30 % ;单独使用IL 12有一定的免疫保护作用。结论 :Sj31BIN +IL 12能诱导小鼠产生较强的抗生殖免疫作用。
Objective: To prove that Schistosoma japonicum recombinant proteinase B nucleic acid vaccine Sj31BIN has the anti-reproductive effect, combined with IL 12 to observe whether IL 12 has an adjuvant effect. Methods: Balb / C mice were immunized with Sj31BIN + IL12 and IL12, respectively. Six weeks after the challenge, the number of adult worms and the number of intra-tissue eggs and the number of liver-surface egg and nodules were counted. Results: Sj31BIN + IL 12 immunized mice reduced the rate of adult development. The reduction rate of liver tissue was 59.74%, that of intestinal tissue was 59.6%, and that of hepatic egg was 71-30%. IL-12 alone had some protective effect. Conclusion: Sj31BIN + IL 12 can induce mice to have a strong anti-reproductive effect.