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观察肾综合征出血热（HFRS）地鼠肾细胞Ⅱ型灭活疫苗在以家鼠型为主疫区接种后一年的血清学和防病效果。方法：采用IFAT和MCPENT法检测疫苗接种者静脉血的荧光抗体和中和抗体阳转率与GMT滴度，追踪观察防病效果。结果：（1）加强免疫前、加强免疫后14天及12个月，IFAT阳性率分别为62.06％、100％和56．1％，GMT分别为18.77、98．83、29.22；中和抗体阳转率分别为18．5％、100％和60％，GMT分别为3．2、17．05和5．75。（2）加强免疫后接种组无一病例；对照组发病12例，发病率为101．2／10万，保护率100％。（3）采集接种人群流行高峰期前（9月）、后（翌年1月）双份血，IFAT未检测出汉坦病毒（HV）自然感染者。结论：加强注射后抗体滴度迅速升高，中和抗体和荧光抗体阳转率均达100％。加强免疫后1年，抗体滴度和阳转率均明显下降，但仍有好的防病效果。
To observe the serological and disease control effects of HFRS hamster kidney cell type II inactivated vaccine one year after inoculation in the home-based mouse-type epidemic area. Methods: The IFAT and MCPENT methods were used to detect the positive rate of antibody and neutralizing antibody positive rate and GMT titers in the venous blood of vaccinated patients. The effect of anti-disease was observed. Results: (1) The positive rate of IFAT was 62.06%, 100% and 56.1% respectively at 14 days and 12 months after booster immunization, and the GMT was 18.77, 98.83 and 29.22 respectively. Turnover rates were 18.5%, 100% and 60% respectively, with GMTs of 3.2, 17.05 and 5.75 respectively. (2) None of the inoculation groups after booster immunization; in the control group, 12 cases were found, the incidence rate was 101.2 / 100000 and the protection rate was 100%. (3) Blood samples were collected before peak sepsis (September) and after (peaked in January), IFAT did not detect natural infection with Hantaviruses (HV). Conclusion: The titer of antibody increased rapidly after injection, and the positive rate of neutralizing antibody and fluorescent antibody reached 100%. One year after booster immunization, antibody titers and positive rate were significantly decreased, but there was still good anti-disease effect.
